FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35088651
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1266970.361076862145541No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1010480.28797915314555694No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC679650.19369510671698378No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT475220.13543410375052606No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT405880.11567272848420418No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC398850.11366923168405647TruSeq Adapter, Index 4 (100% over 50bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT394300.1123725161163933No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA381920.10884430980261965No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA380260.10837122236474694No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC376240.1072255527862841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG94750.031.85866542-43
TATGCCG86800.026.43130744-45
ATGCCGT123700.024.57545346-47
TCTCGTA113200.024.12751840-41
AATCTCG122450.022.01396838-39
GTATGCC140850.021.66743744-45
GCCGTCT144700.020.81191348-49
GAGTCTT506250.020.2759384
GGAGTCT521550.020.182043
TGGAGTC518300.019.9695032
AGTCTTG525900.019.9518785
CGTACGG13350.019.213383
CGTATGC121250.019.15659742-43
CGCACTA506200.019.07215182-83
GTCTTGG554750.018.9313986
CGATCGG540150.017.85582470-71
ATAGCGC548550.017.71661278-79
CGGCATA551250.017.57813674-75
ATCGGCA565900.017.16084572-73
AGCGCAC572900.017.07138680-81
Produced by FastQC (version 0.11.5)