FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28863773
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1643780.5694958867643534No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1316030.45594524319464397No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1220680.4229107539059429No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA509430.1764945975704562No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT508730.17625207903346524No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT476950.16524173745407433No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT448430.1553608393469558No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC431710.14956811086339958No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG343560.11902809795517723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTT459000.029.7209514
TGGAGTC464600.029.689872
GGAGTCT471300.029.2577253
GTCTTGG481300.028.7682616
AGTCTTG480350.028.7658275
TCTTGGA547850.025.8025257
CGCACTA560150.025.08754582-83
CTTGGAA554350.024.908758
CGATCGG560750.024.89128770-71
CCTTAGG663800.024.6887821
TTGGAAG559150.024.5759939
ATAGCGC572200.024.57167678-79
CGGCATA573500.024.42487374-75
AGCGCAC586400.024.00096180-81
CCCGATC585550.023.88978668-69
ATCGGCA592200.023.6175172-73
CTCCGTT237550.023.5031741
CAGAACT601350.023.15546494-95
CACCCGA609000.023.07128566-67
GCATAGC636400.022.18245376-77
Produced by FastQC (version 0.11.5)