FastQCFastQC Report
Thu 2 May 2024
EGAF00005494058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36800279
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1077790.29287549694935733Illumina Single End PCR Primer 1 (100% over 50bp)
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT534010.14511031288648654No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT522220.14190653282818863No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA394540.1072111436981225No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA389060.10572202455312905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)