FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29601967
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC66500.035.79352
GGGCGAT69450.034.619431
TGGCGCG172900.026.3518873
GGCGCGT190800.023.875154
CGATCTG106250.022.5324024
GGTCGAC74300.022.3831331
GGTGGCG213750.021.7852021
GCGATCT112350.021.3553333
GCGCGTG238200.019.3435385
CGCGTGC238550.019.1359426
GTGGCGC243850.018.937892
GCGTGCC267000.017.0969267
TTGAACG61150.016.8565677
TGCGACA124300.016.8146366
GCGACAT127950.016.4834697
AGGTCGA69300.016.1765046
CGACCCG107300.015.7157294
CGTGCGG112150.015.4595659
ACCCGTG112450.015.0382196
CTGCGAC145850.014.5256095
Produced by FastQC (version 0.11.5)