FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27526310
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC76350.023.769241
GGCGATC39600.023.7527352
TGGCGCG120350.023.249373
GGCGCGT125050.022.2977854
TGCGCGA101500.022.2289169
TAACACG107900.019.8563182
TGCGACA68450.018.8756186
GCGACAT68750.018.7240227
GGGCGAT55050.018.6404931
AACACGT117850.018.4216543
GTGCGCG123700.017.8943398
ACACGTG138050.016.1721884
GGTGGCG178500.016.1019361
ACGTGCG140200.015.5514326
CGCGTGC185400.015.3725996
CTGCGAC85300.015.3697145
GCGTGCC184450.015.2713927
CGATCTG67750.014.6535994
CGCGAGT81100.014.08591410-11
GCGCGTG203950.014.0675655
Produced by FastQC (version 0.11.5)