FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33978655
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT742280.2184547916920196No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG494890.14564731888298696No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC397510.11698815035498021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG21450.037.8469243
GCGTACG24800.032.734542
ACCGCGT39450.028.1549956
GTACGGA47350.026.433671
GGCGTAC37200.024.528251
CCGCGTT50950.022.0795787
CGCGTTC51700.021.7592758
CGCACTA205650.021.55349582-83
TGGAGTC370650.021.528882
GAGTCTT371850.021.266774
AGACCGC53950.020.9780854
AGTCTTG401850.020.2339175
TCGTATG56150.020.20387842-43
TATGCCG48600.020.16835246-47
GGAGTCT408200.019.7824253
TACGGAA63700.019.4913922
CGATCGG230200.019.28968470-71
GATGCGT101450.018.9697654
ATAGCGC236550.018.89226378-79
CGGCATA235900.018.8336574-75
Produced by FastQC (version 0.11.5)