FastQCFastQC Report
Wed 1 May 2024
EGAF00005494065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27124749
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG8035842.9625490728043236TruSeq Adapter, Index 19 (97% over 39bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT955820.352379297592763No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT923850.34059301341369097TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAACTCGTATG652580.24058471471938783TruSeq Adapter, Index 19 (97% over 39bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG479230.17667628924418802No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC431960.1592493998746311No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG401800.14813040297626348No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGACCTCGTATG292300.1077613658286755TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)