FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27124749
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG8035842.9625490728043236TruSeq Adapter, Index 19 (97% over 39bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT955820.352379297592763No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT923850.34059301341369097TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAACTCGTATG652580.24058471471938783TruSeq Adapter, Index 19 (97% over 39bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG479230.17667628924418802No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC431960.1592493998746311No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG401800.14813040297626348No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGACCTCGTATG292300.1077613658286755TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC1781550.055.6848079
GAGCACA1821450.054.4543728
AGAGCAC1859400.053.383827
AAGAGCA1994150.049.838426
GAAGAGC2036600.049.0326775
CGGAAGA2053550.048.5268943
TCGGAAG2061150.048.38042
ATCGGAA2117100.047.1427351
TCGTATG1180750.045.7137344-45
GGAAGAG2203900.045.324734
ATGCCGT1179500.045.20481548-49
GCCGTCT1174900.044.81821450-51
TCTCGTA1026800.044.8153142-43
GTATGCC1206100.044.75881646-47
TGAAACG1233900.043.82732834-35
CGTCTTC1211650.043.34916352-53
GATCTCG1064550.043.02324340-41
CACGTGA1265200.042.87251330-31
GTCACGT1259800.042.86035528-29
TATGCCG1173850.041.60720446-47
Produced by FastQC (version 0.11.5)