FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27124749
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT9236933.4053513269376245Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG960090.3539535057080159Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA278930.10283228795960472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1715450.057.534599
AGAGCGT1745200.056.4836967
GAGCGTC1775500.055.430598
AAGAGCG1828000.054.133066
CGGAAGA2035350.048.8001943
TCGGAAG2068100.048.055492
GAAGAGC2130550.046.670975
ATCGGAA2148300.046.418381
CGTATCA1157650.045.5915648-49
TCGCCGT1170250.044.76381344-45
GTGGTCG1186950.044.34852240-41
GGTCGCC1197950.044.08160842-43
GCCGTAT1217650.043.21243746-47
CTCGGTG1238100.043.1434136-37
CGGTGGT1244200.042.59412838-39
GTAGATC1285450.042.42183330-31
ATCTCGG1283300.042.20842434-35
GGAAGAG2376700.041.8660164
AGATCTC1312700.041.2520932-33
CGCCGTA1144200.041.04986244-45
Produced by FastQC (version 0.11.5)