FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27819549
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG619720.22276421519270498Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA125450.034.11457448-49
GCCGTAT126550.033.81813446-47
TCGCCGT146450.029.33640944-45
GGTCGCC159150.027.26410542-43
CGCCGTA115050.027.24860646-47
GTGGTCG162750.026.82210440-41
CCGTATC127200.024.64578448-49
GGCGCGT126850.024.0095351
CGGTGGT193750.022.58008638-39
GGGCGAT50500.022.11021
GGCGATC49750.021.8697852
TGGTCGC147750.021.25021442-43
CTCGGTG207650.021.1486536-37
ATCTCGG232650.019.42734134-35
CGCGTGC157750.019.2758143
GTAGATC230450.018.95286630-31
ACGTGCG49650.018.6556226
TGGCGCG132050.018.2779483
GCGCGTG167250.018.1525172
TCGGTGG178350.017.7781238-39
Produced by FastQC (version 0.11.5)