FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27001925
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1422600.526851326340622TruSeq Adapter, Index 7 (100% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT937820.34731597839783646No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG495460.1834906215019855No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG495000.1833202632775256No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC458430.16977678443296174TruSeq Adapter, Index 7 (100% over 50bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC429010.158881264946851No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA322880.1195766598122171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG247450.040.77133642-43
ACCGCGT62250.039.6015246
ATGCCGT260650.038.32458546-47
TCTCGTA247750.036.93516540-41
GCCGTCT273650.036.36507848-49
GTATGCC283250.035.7947244-45
CGCGTTC72400.034.3122028
AGACCGC73100.034.178444
CCGCGTT74600.033.3001947
CAGACCG77450.032.8107573
CGTCTTC305950.032.32390250-51
CATCTCG284950.032.16331538-39
TATGCCG243700.031.8485244-45
GACCGCG81650.030.3085675
GCGTTCT82150.030.297679
CGTATGC275050.028.28721242-43
TGCCGTC279200.027.46746646-47
ATCTCGT254650.027.13030838-39
CCGTCTT285100.026.70743848-49
TGGAGTC366800.026.6113172
Produced by FastQC (version 0.11.5)