FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26642837
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT372360.13975989118576224No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT364960.1369824091931351No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG337930.12683709321195788No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA294410.11050249641207503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT63900.049.5062686
CCGCGTT71900.043.997927
AGACCGC74700.042.4123234
CGCGTTC75300.042.0743758
GACCGCG80200.039.503755
CAGACCG84000.038.169053
GCGTTCT83250.038.056469
AATACGG78250.022.67216932-33
GAACGAT70300.022.66856444-45
TACGGAG78650.022.34548834-35
CAATACG74600.021.90314330-31
GCAACGC76550.020.88050380-81
ATACGGA78000.020.6134632-33
AACGATC86600.020.2392946-47
CGATCAT89400.019.73822648-49
CGATTAG27700.019.719914
AGAACGA89150.019.71365744-45
CAACGCC90450.019.43112282-83
ACGATCA86050.018.76786446-47
GTTCGAT31850.018.4952141
Produced by FastQC (version 0.11.5)