FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26642837
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT290020.10885477398671921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC114100.023.2306371
ACGTGCG135650.022.8307826
AACACGT149700.020.9105953
TGCGCGA149650.020.8220049
GGCGATC38700.020.0069942
TAACACG157200.019.6108932
TTGAACG49250.018.517747
GTGCGCG175450.018.409888
TGGCGCG120000.018.24833
CCGACCG94000.018.0903997
GGCGCGT124250.017.7767334
CGTGCGC185450.017.1097077
CGCGTGC173250.017.0267161
ACACGTG185200.016.8764214
GGTCGAC34350.016.456321
CTAACAC209100.016.0157621
GCGACAT62150.015.05629357
TCTAACA183000.015.0550472
GCGTGCC195400.015.047742
GAACGCT60850.014.83161459
Produced by FastQC (version 0.11.5)