FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34468011
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA384500.11155270897412677No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT375840.10904023443650404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT94800.040.858576
CCGCGTT100400.038.5323267
AGACCGC112350.034.687374
CAGACCG119350.033.0904163
GCGTTCT120650.032.2617459
GACCGCG129300.030.1768995
GTCGGTT68900.029.2272171
CGCGTTC148550.026.5539848
CGGTTCG69900.026.4843163
TCGTATG81400.023.12175442-43
TATGCCG72850.022.18650446-47
GTTCGGT74150.022.0215875
TACGGAG115650.021.6308834-35
AACGATC114800.021.60457446-47
AATACGG115600.021.59918832-33
ATGCCGT89250.021.24759946-47
GTTCGAT34850.020.8511241
GGTTCGG80650.020.5410394
TCGCTAT125300.020.1919127
CTCGCTA126650.019.9392016
Produced by FastQC (version 0.11.5)