FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36101874
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT533980.14790921933858614No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC526030.14570711758619512No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT484890.13431158725998546No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC472080.13076329500235917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG85100.053.9733772
CGTACGG87700.052.3732533
GGCGTAC94750.048.6962971
GTACGGA115350.039.777934
TACGGAA128200.035.6796765
ACCGCGT33900.033.7675866
GATCGTT94000.029.863615
CCGCGTT40500.028.4992797
TGATCGT100900.027.9155484
AGACCGC47900.024.096474
ATCGTTT128550.022.1698026
TCGTAAC52050.020.89938588-89
AGTCGTA52850.020.44820286-87
CGCGTTC58700.020.2294738
GTTTGAC296100.019.6828885
GCGTTCT60300.019.6139349
TCGTTTT151300.019.0246357
AAGTGCG136800.018.6453636
GTCGTAA56950.018.5590286-87
CGTTTTT164150.017.3906678
Produced by FastQC (version 0.11.5)