FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32777991
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG359260.10960403277919016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT54300.034.5528036
CCGCGTT61800.030.4363617
AGACCGC66950.027.8822634
TCGTATG81150.026.60304842-43
CAGACCG77250.025.0254543
GCGTTCT82950.022.8477029
ATGCCGT102700.021.29831546-47
GACCGCG89600.021.0989635
TCTCGTA94600.020.98800540-41
CGCGTTC95750.019.8429958
CTCGCTA99550.019.037846
TATGCCG70800.018.95270746-47
TCGCTAT100500.018.9524067
GTCGGTT37200.018.5176541
GCCGTCT118600.018.12257848-49
GTATGCC123050.018.06551244-45
AATCTCG115400.017.45203838-39
CGATTAG31700.017.2315624
CGCTATG112400.016.903628
GTTCGAT38800.016.6520651
Produced by FastQC (version 0.11.5)