FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29362286
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG363190.12369268523574764Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC49900.029.7010382
GGGCGAT55000.027.811431
GCCGTAT84900.027.4424146-47
CGTATCA88400.026.4633348-49
TGGCGCG137500.024.1480273
CGCCGTA80100.023.51265146-47
TCGCCGT105200.022.4404544-45
GGCGCGT147800.022.3042584
GGTCGAC51800.021.7344551
CCGTATC90500.020.94184348-49
GGTCGCC118300.020.19651242-43
CGCGTGC173250.019.0552356
GTGGTCG125700.019.02662740-41
GCGCGTG175350.019.0166535
TGGTCGC107350.017.98670842-43
CGATCTG89150.017.1568414
CGACCCG72100.017.0634654
GGTGGCG207300.017.049191
GCGATCT94200.016.5902233
GTGGCGC210550.016.5824872
Produced by FastQC (version 0.11.5)