FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34167572
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA500840.1465834329697176No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT412440.12071094779576377TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT119250.041.9031456
CGTACGG17700.040.5221943
CCGCGTT127050.039.2558067
AGACCGC132750.037.6418044
CAGACCG142250.035.7957733
GCGTACG20750.034.5659182
GCGTTCT144850.034.5629929
CGCGTTC171850.029.2985138
GTCGGTT85350.028.0648211
TCGTATG115500.026.5462744-45
ATGCCGT125950.024.64544548-49
AATACGG139350.024.15027832-33
TACGGAG140450.023.82585334-35
AACGATC142150.023.60774846-47
CGGTTCG107500.022.932333
GTTCGGT94050.022.8280535
GGCGTAC36000.022.5750561
GTACGGA33400.021.7587644
GCCGTCT141750.021.73082250-51
TCGACCC82850.021.4994833
Produced by FastQC (version 0.11.5)