FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31460078
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG337400.1072470322546562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT50000.026.7024631
GGCGATC53100.024.0690882
GGCGCGT155400.023.3897421
GGTCGAC73500.022.1728631
TGGCGCG139600.021.9521053
GAGATAC133200.020.2246363
AGGTCGA61750.019.9231686
GGTGGCG161900.019.457261
GTCGACC102350.018.8938981
CGTATCA45600.018.64559248-49
CGCCGTA35350.018.6099846-47
AGATACC146450.018.358354
GATACCA148150.017.7624845
TATACGC56000.017.642857
GGAGATA165000.017.3922142
TCGACCC114950.016.6983782
GCGTGCC219250.016.4657174
TACGCTA59650.016.4037869
GTGGCGC191300.016.2181052
ATACCAT165150.016.077886
Produced by FastQC (version 0.11.5)