FastQCFastQC Report
Wed 1 May 2024
EGAF00005494099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25594478
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT649770.25387116705408097TruSeq Adapter, Index 16 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT352590.13776018405220064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)