FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25594478
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT649770.25387116705408097TruSeq Adapter, Index 16 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT352590.13776018405220064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG107300.032.7804344-45
TATGCCG101200.032.0574948-49
ATGCCGT119150.029.60000248-49
TCTCGTA119300.027.57202542-43
CGTATGC120300.027.2441246-47
GCCGTCT132900.026.43032650-51
CCGATCT127350.025.6240138-39
ACCGCGT25050.025.598556
TCCCGAT131950.024.964736-37
GTATGCC144750.024.56193546-47
ATCTCGT126500.024.16280742-43
TGCCGTC135250.024.05706450-51
CCGTCTT142000.022.61245352-53
GTCCCGA146950.022.01237336-37
CGTCTTC161750.021.70148752-53
CCGCGTT30050.020.8650197
ACCCGTC158800.020.56418432-33
CGTCCCG175150.020.40730734-35
GATCTCG165550.020.156140-41
CGCACTA93950.019.768382-83
Produced by FastQC (version 0.11.5)