FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25594478
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG701470.2740708366859445Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT105650.033.3820846-47
CGCCGTA98400.033.16256346-47
CGTATCA106400.033.035248-49
CCGTATC108400.029.9280448-49
TCGCCGT128700.027.47731444-45
TGGTCGC128750.025.43771642-43
GTGGTCG144350.024.63031440-41
GGTCGCC150350.023.66298142-43
ACGTGCG57450.021.0009546
TCGGTGG163150.020.35150138-39
TCTCGGT164450.020.17619736-37
CGGTGGT183950.019.63838438-39
GGCGCGT77550.019.6055811
GTATCAT175800.018.89973350-51
CTCGGTG195400.018.48763836-37
TGCGCGA70300.017.7699939
TAACACG66500.017.5755842
GGCGATC36750.017.4531062
ATCTCGG212700.017.36361534-35
GTCTAAC65500.016.9740771
Produced by FastQC (version 0.11.5)