FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26684062
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1661890.6228024803719913TruSeq Adapter, Index 13 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT945270.3542451670214228No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG695270.260556282622938No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC505580.1894689046967437No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG479380.1796503096117825TruSeq Adapter, Index 13 (97% over 39bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG455990.17088477758746026No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA387970.14539390592032053No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA374540.14036093905043392No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC292790.10972467385212942No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG268230.10052067784882227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG285050.040.41771344-45
ATGCCGT293800.038.89063648-49
ACCGCGT66600.038.6559686
TCTCGTA283600.037.08197842-43
GTATGCC313250.036.84738546-47
GCCGTCT314150.036.1521450-51
AATCTCG306350.034.32046540-41
AGACCGC77900.033.3534974
CGTCTTC341900.033.0928552-53
CCGCGTT81400.031.7443187
CAGACCG83050.031.6283843
TATGCCG275900.031.46278648-49
CGCGTTC86100.029.9562978
GCGTTCT88700.029.0246589
ATCTCGT291800.028.25894442-43
CGTATGC321750.027.16381646-47
TGCCGTC319600.027.16077250-51
CCGTCTT326000.026.4818552-53
GTCAACA438100.026.47128134-35
GCTTGAA448350.026.10447960-61
Produced by FastQC (version 0.11.5)