FastQCFastQC Report
Wed 1 May 2024
EGAF00005494103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26432499
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT572370.21654025220997833No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG390080.14757590646272228No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT343150.12982124769965944TruSeq Adapter, Index 15 (97% over 40bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC313070.11844131725872759No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT278770.1054648673210959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)