FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26432499
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT572370.21654025220997833No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG390080.14757590646272228No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT343150.12982124769965944TruSeq Adapter, Index 15 (97% over 40bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC313070.11844131725872759No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT278770.1054648673210959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT33500.030.2013386
CCGCGTT42950.023.2245627
TCGTATG89500.023.1130144-45
ATGCCGT91800.022.63741148-49
AGACCGC45850.021.7556154
TATGCCG79750.021.7099248-49
CGCACTA154600.021.307382-83
TGGAGTC281500.020.5691682
GAGTCTT276400.020.450334
CAGACCG51650.020.0483023
GTCTTGG285100.019.9928846
CGGCATA173200.019.18365174-75
AGTCTTG301350.019.135465
GCCGTCT109350.019.06940550-51
TCTCGTA100600.018.95740942-43
CGCGTTC52650.018.9457728
GGAGTCT311000.018.8471723
ATAGCGC176300.018.8463378-79
ATCGGCA180150.018.48311472-73
GATGCGT66750.018.1459754
Produced by FastQC (version 0.11.5)