FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26432499
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG365060.13811028612920784Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG92150.026.9589736
TGCGCGA102550.024.8271069
GCCGTAT87400.023.7498746-47
AACACGT105600.023.7068183
CGTATCA88550.023.62922148-49
TAACACG104200.023.432682
GGCGCGT114400.022.630951
CGCCGTA79450.021.6723246-47
GTCTAAC83150.021.3097741
TGCGACA94050.021.1112776
GCGACAT95850.020.8139327
GGCGATC45100.020.2235321
ACACGTG127550.019.8864754
CCGTATC91350.019.05712948-49
TTACAGC169900.018.453432
GTGCGCG138100.018.2984778
TCGCCGT114300.018.26433644-45
CGACATC111950.018.1176058
CGTGCGC140600.017.9731127
CTAACAC151550.017.8669831
Produced by FastQC (version 0.11.5)