FastQCFastQC Report
Wed 1 May 2024
EGAF00005494105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28571989
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1257300.44004636849048206No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1055000.3692427573033155TruSeq Adapter, Index 12 (100% over 50bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG581840.2036400056012901No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC485030.16975717021310627No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC472650.1654242552032342TruSeq Adapter, Index 12 (100% over 50bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG370560.1296934560628593No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA287090.1004795290940368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)