FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28571989
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1257300.44004636849048206No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1055000.3692427573033155TruSeq Adapter, Index 12 (100% over 50bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG581840.2036400056012901No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC485030.16975717021310627No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC472650.1654242552032342TruSeq Adapter, Index 12 (100% over 50bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG370560.1296934560628593No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA287090.1004795290940368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG223950.037.0538442-43
ATGCCGT240500.034.45460546-47
ACCGCGT41050.033.5564656
TCTCGTA234150.032.99523540-41
GCCGTCT248550.032.89914348-49
GTATGCC258700.032.32443644-45
AATCTCG241200.031.92251238-39
CCGCGTT43850.031.6303927
CGCGTTC46700.029.5983498
CGTCTTC286900.028.542950-51
TGGAGTC457300.028.0241282
GAGTCTT456650.027.9808044
GGAGTCT471150.027.3414693
AGACCGC51900.026.99894
GACCGCG51600.026.8797055
AGTCTTG487350.026.6665295
GTCTTGG498800.025.8544186
TATGCCG215950.025.38305946-47
CTTGGAA546200.023.3411468
TTGGAAG554050.023.036169
Produced by FastQC (version 0.11.5)