FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28571989
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1117550.3911348278903509Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT540710.1892447879634841Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA447920.15676892497753656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA216000.038.0548548-49
GCCGTAT218150.037.74511746-47
TCGCCGT246450.033.54574244-45
GGGCGAT59050.033.2252271
GGCGATC68700.032.776261
GGTCGCC260750.032.12504242-43
GTGGTCG269250.031.06680540-41
CGGTGGT293700.028.60993238-39
CTCGGTG305750.027.5522936-37
ATCTCGG318900.026.71403134-35
GGCGCGT173100.026.7025991
CGCCGTA212850.025.7751146-47
CCGTATC218950.025.02446648-49
GTAGATC344500.024.37038630-31
TCATTAA381700.022.31260752-53
TGGTCGC250250.022.046442-43
TATCATT384850.021.80907650-51
AGATCTC393300.021.55186332-33
CGCGTGC214100.021.3444793
TGCGACA209850.021.277076
Produced by FastQC (version 0.11.5)