FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26921776
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1042920.38738900435097595No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA737850.2740718145786519No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG536760.19937763392727137No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG533360.19811471576020842No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA433840.16114835811723566No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC428410.15913140351513214No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT306930.11400808029901148No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC285960.1062188467803907No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC275190.10221836776295888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT121850.059.993556
AGACCGC131000.056.093234
GACCGCG133350.055.1403355
CAGACCG137300.053.5539933
CCGCGTT139400.052.4064877
CGCGTTC146300.049.934828
GCGTTCT159250.046.2022829
TCAGACC246750.031.0120052
CTCAGAC288650.028.8039681
GAGTCTT388950.027.2456574
TGGAGTC394500.026.8382722
AACGATC149900.026.04723546-47
GGAGTCT409050.025.9649143
AGTCTTG411100.025.87015
GTCTTGG413350.025.8441936
GAACGAT147000.025.75327544-45
AATACGG154450.025.3875432-33
TACGGAG153950.025.36200534-35
CAATACG151550.025.07410830-31
ATACGGA152750.024.95486832-33
Produced by FastQC (version 0.11.5)