FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26921776
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT416340.15464804402205856No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA339600.12614323809840777No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT274870.10209950487664707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA129400.026.7233856
GCGACAT132450.026.3231877
CTGCGAC148500.023.5101435
GGCGATC47250.023.4250182
GCTGCGA151300.023.1378464
GGGCGAT49250.022.6667421
CGACATC154250.022.5105348
CGCGTGC200050.020.5639631
GGTCGAC49600.020.3041
GCGTGCC220600.018.5405882
GGCGCGT129250.018.3031671
GGCTGCG215850.016.4836433
TCGACCC66850.016.1305922
GTCGACC69250.015.9831951
ACGTGCG43500.015.7241676
TGGCGCG141050.015.3910752
CGTGCCT276150.014.9485193
GGCGTAG41450.014.26716134-35
GCGCGTG174250.013.8216862
CGACCCG80900.013.74012853
Produced by FastQC (version 0.11.5)