FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26912390
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT839670.31200127524905813No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA543330.20188842388208553No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG415690.15446045483139922No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG395080.14680227211332772No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA335470.12465262282539752No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC330800.12291736259767341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT89200.059.907266
CCGCGTT100750.053.1337787
AGACCGC101200.052.7566954
GACCGCG101050.052.7409975
CAGACCG107600.049.795333
CGCGTTC107650.049.5957078
GCGTTCT120950.044.45629
TCAGACC205250.027.4237582
CTCAGAC245300.025.6238541
GAGTCTT349700.024.829754
TGGAGTC346950.024.725362
GGAGTCT353650.024.538993
GAACGAT115250.024.23417344-45
GTCTTGG366200.023.9444686
GCAACGC115800.023.93448480-81
AATACGG119100.023.82966432-33
TACGGAG119550.023.64063834-35
AGTCTTG371800.023.5710435
ATACGGA118750.023.43990132-33
AACGATC126050.022.40272346-47
Produced by FastQC (version 0.11.5)