FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26912390
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT320280.11900838238447049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA103500.027.2150486
GCGACAT104550.027.1688947
CGACATC121900.023.301958
CTGCGAC121350.023.2901345
GCTGCGA131000.021.5019684
CGCGTGC157950.021.0229821
GGTCGAC54800.020.71831
GGCGATC36600.018.041411
GCGTGCC184250.017.9185452
TCGACCC67250.017.5886173
GGCGCGT100950.017.5520233
ACGTGCG41150.017.1993186
GTCGACC70300.016.622842
GGCTGCG171950.016.6033973
TCACCCG74650.015.9087063
TGGCGCG111400.015.8629242
CGTGCGG77900.015.243869
ACCCGTG81800.014.92363556
TGCGCGA50700.013.8658389
CCGTGCG85850.013.8323038
Produced by FastQC (version 0.11.5)