FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32113982
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3809691.1863025893207513Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1056280.32891592204292824Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA427530.13312892807874152No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG332260.10346272224976648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA543100.043.39787748-49
GCCGTAT545000.043.2422346-47
TCGCCGT576100.041.00679444-45
GGGCGAT81600.039.7630421
GTGGTCG602850.039.49081440-41
GGTCGCC626500.037.9884542-43
CGGTGGT636750.037.41073638-39
ATCTCGG648000.036.8234934-35
CTCGGTG650000.036.6298636-37
GTAGATC674800.035.2834930-31
CGCCGTA538900.034.28727746-47
CCGTATC548800.033.66875548-49
GGCGATC93700.033.6638152
TCATTAA709450.033.60703752-53
TATCATT721500.032.93054650-51
AGATCTC727950.032.82478332-33
GAGCGTC1133600.032.6164939
GAGTGTA731250.032.527226-27
TGGTCGC580850.031.92138742-43
AGAGCGT1178600.031.3268348
Produced by FastQC (version 0.11.5)