FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005494119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31770086
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG5290941.6653842233854825TruSeq Adapter, Index 19 (97% over 39bp)
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA519460.16350601002465023No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAACTCGTATG440060.13851394673593267TruSeq Adapter, Index 19 (97% over 39bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT422940.1331252298152419No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG335600.1056339601976526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)