FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31504348
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG37000.043.4910623
ACCGCGT48500.040.4213076
GCGTACG40500.039.9669842
CCGCGTT50900.038.4221347
GGCGTAC51950.032.636011
GTACGGA53150.030.4546184
AGACCGC65550.029.762624
GCGTTCT77000.025.6451159
CGCGTTC80750.024.9832218
TACGGAA65450.024.8038165
GTCGGTT48150.023.07991
CGATTAG25750.021.752384
CGGTTCG31850.021.6102893
TCGATTA29450.019.9865763
GATCGTT48550.019.8476375
TCGCTAT95350.019.3158177
CTCGCTA96950.018.997046
CGCTATG101650.018.1186738
TGATCGT53500.017.8338174
AATACGG64200.017.745532-33
Produced by FastQC (version 0.11.5)