FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31504348
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC87200.027.4644261
TGGCGCG133500.021.1749233
GGCGATC40950.020.8837342
GTCTAAC104200.019.7458881
GGCGCGT157400.018.1070564
GTCGACC130450.018.064552
TCGACCC134000.017.8340233
CGTATCA52700.017.66559248-49
CGACCCG146600.016.8812684
GGGCGAT53200.016.613371
ACCCGTG154550.016.0436386
AGGTCGA75100.016.0022816
CGCCGTA45700.015.69434346-47
GCGTGCC187600.015.2174887
CCGTGCG161000.014.8698068
TAACACG126300.014.47967154
CGTGCGG168550.014.0063239
TTCCGCA56050.013.8165033
AACACGT133350.013.6429115
GGTGGCG216300.013.6204561
Produced by FastQC (version 0.11.5)