FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32333870
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT463690.14340689809169146No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG413380.12784736253346723No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC328710.10166119923164163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT41300.041.8350266
CCGCGTT45800.037.8282367
CGCGTTC49150.035.0567638
GACCGCG50650.034.3934175
AGACCGC56500.030.5108494
TCGTATG61650.024.33003442-43
CAGACCG72850.024.2531243
GCGTTCT76300.023.142339
CGATGCG90150.022.4063013
TATGCCG54650.020.93223446-47
CGCACTA177750.020.93194282-83
GATGCGT96650.020.6523724
ATGCCGT76250.019.98268746-47
TATCTCG73950.019.54509738-39
CGATCGG193850.019.13603270-71
ATGCGTG106350.018.7902835
CGCCAAT83550.018.46399332-33
TCGATGC116050.018.310932
CGGCATA207650.017.92143874-75
ATAGCGC209400.017.89633878-79
Produced by FastQC (version 0.11.5)