FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26957181
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT640190.23748403069297194No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG376070.1395064268774988No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA353440.131111632184389No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT351090.1302398793108226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT69300.053.120186
CCGCGTT77700.047.438597
AGACCGC80650.045.8211864
GACCGCG82500.044.793685
CGCGTTC86300.042.9864238
GCGTTCT92950.039.859919
CAGACCG97100.038.449833
TACGGAG86400.023.74999234-35
AATACGG87550.023.43798432-33
GAACGAT81450.023.00639544-45
GCAACGC83500.022.4983780-81
ATACGGA86300.021.93361332-33
CAACGCC92950.021.92294982-83
CAATACG87050.021.85375230-31
CGTACGG9600.021.7707063
GAGTCTT310150.021.762734
AACGATC94400.021.5359346-47
AGTCTTG333000.021.0254025
TGGAGTC328800.020.9328162
CGATCAT99050.020.66873248-49
Produced by FastQC (version 0.11.5)