FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26957181
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC46400.026.3110922
GGGCGAT52250.023.911771
TGGCGCG117900.022.5630592
GGCGCGT128200.020.8614333
TTGAACG50750.019.2809477
TGCGACA98400.018.585016
GCGACAT101250.018.5310157
CTGCGAC116100.016.1607695
GCGCGTG167300.016.0984574
CGCGTGC171250.015.7271365
GCTGCGA120550.015.4065964
CGATCTG83650.015.2750814
GTGGCGC180350.015.0404981
GCGATCT89450.014.9227793
ACGTGCG51200.014.6583236
GGTGGCG162500.014.4708531
CGACATC134300.014.1475478
GGTCGAC37350.014.1180481
TGCGCGA55850.013.948199
GCGTGCC197400.013.8362326
Produced by FastQC (version 0.11.5)