FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33951548
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1129500.33267997088085643No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1118510.329443005072994No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1092070.3216554367417945No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT830140.24450726075877305No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA691730.20374034197203614No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC624780.18402106437090882No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA599400.17654570566266964No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC587560.17305838308167865No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG529630.15599583264951572No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA429810.12659511136281623No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC420240.12377638863476859No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT382900.11277836285992027No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT380440.11205380090474815No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG352860.10393045996017619No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG341140.10047848186480333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT203900.056.314596
AGACCGC215900.053.228534
CGCGTTC228500.050.210298
GCGTTCT229300.050.138629
CAGACCG232100.049.7791823
CCGCGTT232400.049.2451447
GACCGCG234100.049.1714065
AATACGG216750.030.25554532-33
TACGGAG221750.029.68035534-35
AACGATC230750.028.50146146-47
AGAACGA232100.028.2743344-45
CGATCAT240100.027.35201548-49
CAATACG213600.027.0013230-31
GAACGAT214800.026.8610544-45
CAACGCC246650.026.53905782-83
ATACGGA220650.026.34316832-33
GTCGGTT65450.026.2678491
AGCAACG253100.025.99401780-81
GCAACGC226750.025.4035780-81
TCAGACC466400.025.31162
Produced by FastQC (version 0.11.5)