FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34350158
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1963510.5716160024649668No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1204570.35067378729378773No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1179490.3433725108338657No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1164530.33901736347180705No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1033880.3009826039228117No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC917910.2672214782825744No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG592500.17248828957351522No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC566590.1649453839484523No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA539170.15696288791451848No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT531730.15479695901253204No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC452550.1317461188970368No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC433790.126284717525899No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC400410.11656714941456747No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA399160.11620325006947566No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT397290.11565885664921834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT202900.059.415616
AGACCGC211900.057.0938074
CAGACCG224250.054.224873
GACCGCG224200.053.961545
CCGCGTT236050.051.0513767
CGCGTTC237400.050.741068
GCGTTCT256450.047.0644459
TCAGACC365050.033.999932
CTCAGAC417800.031.5583651
TGGAGTC638400.031.1233232
GAGTCTT645400.030.7563234
GGAGTCT659600.030.2094143
AGTCTTG678150.029.6422335
GTCTTGG701200.028.694926
CTTGGAA751250.026.5745498
TCTTGGA768250.026.3389247
TTGGAAG755850.026.2431459
AACGATC247550.025.74060446-47
AATACGG246950.025.73582332-33
GAACGAT243000.025.71435244-45
Produced by FastQC (version 0.11.5)