FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28427338
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1337910.47064202775511377No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1023830.3601568321310986No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC567750.19971972050284834No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT565760.19901969013067633No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT436780.15364787234035068No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT345120.12140426233367332No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA334040.11750660578911751No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC305210.10736495974403232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG15750.031.0428583
TGGAGTC446500.030.0863842
GAGTCTT451650.029.5961824
AGTCTTG467600.028.8810395
GGAGTCT465850.028.7653583
GCGTACG18050.027.6132262
GTCTTGG519000.026.0573436
TTGGAAG538900.024.936579
TCTTGGA570850.024.0065547
CTTGGAA582700.023.5020548
TCGCTAT54650.022.67022
CTCGCTA56900.021.3685441
CCTACGT338600.020.42573224-25
TACGTTC339250.020.19777326-27
GTACGGA33250.020.1407051
GACTACC354350.019.49161318-19
CGCTATG61300.019.2816373
CGCACTA394700.019.26639482-83
CTACGTT342050.019.19977626-27
CTGGAGT804250.018.673171
Produced by FastQC (version 0.11.5)