FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28427338
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA379620.13354046727836422No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA285030.10026615928652904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT71550.037.5238761
GGCGATC72000.036.887972
TGGCGCG182800.028.2523773
GGTGGCG200700.026.2338051
GCGATCT111950.024.8699653
GGCGCGT208350.024.7596234
CGATCTG111950.024.6099974
TGCGACA170800.023.138846
GTGGCGC227800.023.1095492
GCGACAT172500.023.048497
CTGCGAC190700.020.923525
GCGCGTG252450.020.5472345
GCTGCGA193900.020.480294
CGCGTGC270550.019.032156
CGGTGGC184950.018.3961751
GCGTGCC286550.018.068927
CGACATC253900.016.014648
GGCTGCG261750.015.3199513
CGTGCCT342150.015.2854038
GCCACGA112300.013.83119218-19
Produced by FastQC (version 0.11.5)