FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31351570
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA387040.12345155282494624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT87150.042.8397186
CCGCGTT93350.039.841797
GACCGCG94850.039.5622675
CGCGTTC97450.038.3117648
AGACCGC98800.037.8363464
CAGACCG104950.035.754943
GCGTTCT112400.033.385069
GTCGGTT49300.025.0558971
CGGTTCG36650.023.9766863
TACGGAG106600.022.56906934-35
AATACGG107150.022.18724332-33
AACGATC109150.021.84597446-47
TCGTATG69400.021.73079942-43
TATGCCG60900.020.47403546-47
CTCGCTA102300.020.2442746
TCGCTAT105200.019.7313657
CAACGCC121650.019.64026382-83
AGAACGA125500.019.05667744-45
CGCTATG110700.018.8797598
GAACGAT102150.018.80925444-45
Produced by FastQC (version 0.11.5)