FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31351570
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC50000.027.2667642
GGGCGAT54150.025.5284561
GCCGTAT64700.023.12591246-47
GGCGCGT123500.022.9999494
TGGCGCG128300.022.437043
CGTATCA67200.021.98283648-49
CGCGTGC145600.019.7046246
CGCCGTA63200.019.6914146-47
GCGCGTG156550.018.478085
CCGTATC68550.018.22388348-49
TCGCCGT85100.017.55430844-45
CGATCTG80750.016.9999624
GGTCGAC45500.016.8091011
GGTCGCC90700.016.7323242-43
GCGTGCC180650.015.8289157
GGTGGCG186850.015.7880521
GTGGCGC186900.015.58019352
GCGATCT97150.014.8645053
GTGGTCG106200.014.4020440-41
TGGTCGC88350.014.32792542-43
Produced by FastQC (version 0.11.5)