FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31084518
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT656320.21114047835646027No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG617050.19850717968346812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT44700.032.1975256
CCGCGTT49800.028.995577
AGACCGC60400.024.0642244
CAGACCG70050.021.6984773
GACCGCG68300.021.2808075
CGCGTTC69500.020.9817148
GCGTTCT77050.018.9873989
GAGTCTT396700.017.074374
CGCACTA269800.016.99866582-83
TGGAGTC414950.016.437892
AGTCTTG424450.016.383325
CGTACGG13650.016.3550783
GTCTTGG427300.016.3185126
GGAGTCT424350.016.1745073
GTTCGAT32800.016.0770511
TCGACCC39900.015.3569222
GTCGGTT28250.014.9667961
CGATCGG308400.014.9321170-71
GCGTACG14700.014.8637322
CGGCATA315250.014.7206774-75
Produced by FastQC (version 0.11.5)