FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32960401
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1271070.3856354781605964No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1183680.35912184442173506No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC668960.20295869579984782No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT592390.17972778911275988No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT591890.17957609192922136No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA570870.17319874233326227No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT556530.16884806710937772No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG432250.1311422151690448No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC379660.11518670540446399No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC359590.10909758045722805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTT458700.025.4635814
TGGAGTC461550.025.398972
AGTCTTG485150.024.231985
GGAGTCT490350.024.0621913
CTCGCTA51800.022.7542271
GTCTTGG519100.022.729526
TCGCTAT52050.021.4455322
TTGGAAG559100.021.103379
TCTTGGA582050.020.7364377
CTTGGAA581900.020.6438278
CGCTATG55550.019.4957753
GTTTGAC173000.019.2469375
CGAAACG66500.019.1426873
TACGTTC322100.018.51465826-27
CGCACTA519400.018.42734782-83
CCTACGT323100.018.39855224-25
GTCGGTT31400.018.1631741
CAGAACT528650.017.99709794-95
CGGCATA536900.017.86210674-75
ATAGCGC537800.017.82779778-79
Produced by FastQC (version 0.11.5)