FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32311558
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA619610.19176110294650603No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC540570.16729926795854289TruSeq Adapter, Index 10 (100% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT470870.14572803948358046No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT454630.1407019741975921No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC352880.10921169446549127No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG336680.10419800865065065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT140100.043.6346666
CCGCGTT153550.039.936297
AGACCGC153450.039.8075454
GACCGCG154100.039.73215
CGCGTTC155100.039.6290558
CAGACCG158750.038.6281363
GCGTTCT165750.037.1114169
GTCGGTT63800.025.840181
CGGTTCG53600.025.7881093
TCGTATG120100.025.6482842-43
AATACGG158100.025.1168832-33
TACGGAG162000.024.48289334-35
TATGCCG107800.024.43284646-47
AACGATC161500.024.3528146-47
ATGCCGT132100.023.49816946-47
CGTACGG11800.022.5422523
TCGCTAT162550.022.383767
AGAACGA176400.022.34965344-45
CTCGCTA162250.022.3080466
CGATCAT181900.021.81750348-49
Produced by FastQC (version 0.11.5)