FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32311558
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG573900.1776144622924094Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC59650.031.0582282
CGTATCA113250.027.15775348-49
GCCGTAT113400.027.10088346-47
GGGCGAT70450.027.0393311
TGGCGCG199700.024.2866423
GGTCGAC73650.023.8005071
GGCGCGT206800.023.3595624
CCGTATC116950.022.70407748-49
CGCCGTA116450.022.69958546-47
TCGCCGT144250.021.48606944-45
CGCGTGC243750.019.896446
GCGCGTG249350.019.4686495
GGTCGCC161550.019.34693542-43
GTGGTCG165300.018.99425340-41
TGGTCGC146150.018.21671542-43
GGTGGCG271750.017.9353731
CGATCTG103500.017.8985794
GCGTGCC278150.017.4528397
TTGAACG71600.017.4476537
GCGATCT118400.016.7303163
Produced by FastQC (version 0.11.5)